What Is A Patterson Map And How Can It Contribute To Protein Structure Determination By X-Ray Analysis? assignment

1. What is a Patterson Map and how can it contribute to protein structure determination by X-ray analysis?2. a) How does an Argand diagram help to understand structure factors in X-ray analysis.b) After X-ray data collection and processing of a heavy atom derivative of a protein crystal you obtain a structure factor magnitude │Fph│of 6 for a particular X-ray reflection hkl. The position of the bound heavy atom determined from a difference Patterson map gives a calculated │Fh│ of 2 and a phase of 45o for the same reflection. The native protein crystal gave │Fp│ of 5. Use a Harker diagram to estimate the phase of this hkl. If you repeated this process for all the measured reflections, would this enable the production of a useful electron density map? If not, what should be done next?3. For a protein containing no -sheet secondary structure the CD spectrum gives [Ө] 222 = -29,750 deg.cm 2. decimole -1. Given from reference spectra that 100% helix has a [Ө] 222 = -36,000 deg.cm 2. decimole -1 and 100% coil has a [Ө] 222 = +3,000 deg.cm 2. decimole -1.a) What is the percentage alpha helical content of the protein? Show your working.b) The protein and a short duplex oligonucleotide are added at equimolar concentration in a buffer containing 100 mM NaCl and then in a buffer containing 1 M NaCl, the two samples are analysed by CD. The spectra in the 250 to 280 nm region differs considerably between the samples but the spectrums in the 200 – 230 nm range are similar. What hypothesis can you form concerning the protein?
Approximate price: $22
We value our customers and so we ensure that what we do is 100% original..

With us you are guaranteed of quality work done by our qualified experts.Your information and everything that you do with us is kept completely confidential.You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.The Product ordered is guaranteed to be original. Orders are checked by the most advanced anti-plagiarism software in the market to assure that the Product is 100% original. The Company has a zero tolerance policy for plagiarism.The Free Revision policy is a courtesy service that the Company provides to help ensure Customer’s total satisfaction with the completed Order. To receive free revision the Company requires that the Customer provide the request within fourteen (14) days from the first completion date and within a period of thirty (30) days for dissertations.The Company is committed to protect the privacy of the Customer and it will never resell or share any of Customer’s personal information, including credit card data, with any third party. All the online transactions are processed through the secure and reliable online payment systems.By placing an order with us, you agree to the service we provide. We will endear to do all that it takes to deliver a comprehensive paper as per your requirements. We also count on your cooperation to ensure that we deliver on this mandate.